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Use the following information to answer the following questions. In 1971, David Baltimore described a scheme for classifying viruses based on how the virus produces mRNA. The table below shows the results of testing five viruses for nuclease specificity, the ability of the virus to act as an mRNA, and presence (+) or absence (-) of its own viral polymerase.  Virus Nuclease  Sensitivity  Genome as mRNA PolymeraseA Dnase +B Rnase +C Dnase +D Rnase +E Rnase +\begin{array}{ll}\text { Virus Nuclease } & \text { Sensitivity } &\text { Genome as mRNA}&\text { Polymerase} \\A & \text { Dnase }&-&+ \\\mathrm{B} & \text { Rnase }&+&- \\\mathrm{C} & \text { Dnase } &-&+\\\mathrm{D} & \text { Rnase }&-&+ \\\mathrm{E} & \text { Rnase }&+&-\end{array} -Given Baltimore's scheme, a positive sense single-stranded RNA virus such as the polio virus would be most closely related to which of the following?


A) T-series bacteriophages
B) retroviruses that require a DNA intermediate
C) single-stranded DNA viruses such as herpesviruses
D) nonenveloped double-stranded RNA viruses
E) linear double-stranded DNA viruses such as adenovirus

F) C) and D)
G) B) and C)

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Which of the following is characteristic of the lytic cycle?


A) Many bacterial cells containing viral DNA are produced.
B) Viral DNA is incorporated into the host genome.
C) The viral genome replicates without destroying the host.
D) A large number of phages is released at a time.
E) The virus-host relationship usually lasts for generations.

F) A) and C)
G) A) and B)

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What characteristics of electron microscopes make them most useful for studying viruses?


A) high energy electrons with high penetrance
B) requirement that specimens be viewed in a vacuum
C) necessity for specimens to be dry and fixed
D) shorter wavelengths providing higher resolution
E) use of magnetic fields to focus electrons

F) A) and D)
G) B) and D)

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Why do RNA viruses appear to have higher rates of mutation?


A) RNA nucleotides are more unstable than DNA nucleotides.
B) Replication of their genomes does not involve the proofreading steps of DNA replication.
C) RNA viruses replicate faster.
D) RNA viruses can incorporate a variety of nonstandard bases.
E) RNA viruses are more sensitive to mutagens.

F) C) and D)
G) A) and D)

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Emerging viruses arise by


A) mutation of existing viruses.
B) the spread of existing viruses to new host species.
C) the spread of existing viruses more widely within their host species.
D) all of the above
E) none of the above

F) None of the above
G) A) and E)

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What is the name given to viruses that are single-stranded RNA that acts as a template for DNA synthesis?


A) retroviruses
B) proviruses
C) viroids
D) bacteriophages
E) lytic phages

F) C) and D)
G) A) and B)

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Which of the following is the best predictor of how much damage a virus causes?


A) ability of the infected cell to undergo normal cell division
B) ability of the infected cell to carry on translation
C) whether the infected cell produces viral protein
D) whether the viral mRNA can be transcribed
E) how much toxin the virus produces

F) B) and E)
G) A) and C)

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Which treatment could definitively determine whether or not the component is a viroid?


A) I
B) II
C) III
D) IV
E) first II and then III

F) B) and E)
G) None of the above

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Which of the following series best reflects what we know about how the flu virus moves between species?


A) An avian flu virus undergoes several mutations and rearrangements such that it is able to be transmitted to other birds and then to humans.
B) The flu virus in a pig is mutated and replicated in alternate arrangements so that humans who eat the pig products can be infected.
C) A flu virus from a human epidemic or pandemic infects birds; the birds replicate the virus differently and then pass it back to humans.
D) An influenza virus gains new sequences of DNA from another virus, such as a herpesvirus; this enables it to be transmitted to a human host.
E) An animal such as a pig is infected with more than one virus, genetic recombination occurs, the new virus mutates and is passed to a new species such as a bird, the virus mutates and can be transmitted to humans.

F) C) and D)
G) A) and B)

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Use the following information to answer the following questions. In 1971, David Baltimore described a scheme for classifying viruses based on how the virus produces mRNA. The table below shows the results of testing five viruses for nuclease specificity, the ability of the virus to act as an mRNA, and presence (+) or absence (-) of its own viral polymerase.  Virus Nuclease  Sensitivity  Genome as mRNA PolymeraseA Dnase +B Rnase +C Dnase +D Rnase +E Rnase +\begin{array}{ll}\text { Virus Nuclease } & \text { Sensitivity } &\text { Genome as mRNA}&\text { Polymerase} \\A & \text { Dnase }&-&+ \\\mathrm{B} & \text { Rnase }&+&- \\\mathrm{C} & \text { Dnase } &-&+\\\mathrm{D} & \text { Rnase }&-&+ \\\mathrm{E} & \text { Rnase }&+&-\end{array} -Based on the above table, which virus meets the requirements for a bacteriophage?

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Which of the following describes plant virus infections?


A) They can be controlled by the use of antibiotics.
B) They are spread throughout a plant by passing through the plasmodesmata.
C) They have little effect on plant growth.
D) They are seldom spread by insects.
E) They can never be inherited from a parent.

F) None of the above
G) B) and E)

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Most human-infecting viruses are maintained in the human population only. However, a zoonosis is a disease that is transmitted from other vertebrates to humans, at least sporadically, without requiring viral mutation. Which of the following is the best example of a zoonosis?


A) rabies
B) herpesvirus
C) smallpox
D) HIV
E) hepatitis virus

F) A) and B)
G) B) and C)

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A researcher lyses a cell that contains nucleic acid molecules and capsomeres of tobacco mosaic virus (TMV) . The cell contents are left in a covered test tube overnight. The next day this mixture is sprayed on tobacco plants. Which of the following would be expected to occur?


A) The plants would develop some but not all of the symptoms of the TMV infection.
B) The plants would develop symptoms typically produced by viroids.
C) The plants would develop the typical symptoms of TMV infection.
D) The plants would not show any disease symptoms.
E) The plants would become infected, but the sap from these plants would be unable to infect other plants.

F) B) and D)
G) A) and E)

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The difference between vertical and horizontal transmission of plant viruses is that


A) vertical transmission is transmission of a virus from a parent plant to its progeny, and horizontal transmission is one plant spreading the virus to another plant.
B) vertical transmission is the spread of viruses from upper leaves to lower leaves of the plant, and horizontal transmission is the spread of a virus among leaves at the same general level.
C) vertical transmission is the spread of viruses from trees and tall plants to bushes and other smaller plants, and horizontal transmission is the spread of viruses among plants of similar size.
D) vertical transmission is the transfer of DNA from one type of plant virus to another, and horizontal transmission is the exchange of DNA between two plant viruses of the same type.
E) vertical transmission is the transfer of DNA from a plant of one species to a plant of a different species, and horizontal transmission is the spread of viruses among plants of the same species.

F) B) and D)
G) A) and E)

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What are prions?


A) misfolded versions of normal brain protein
B) tiny molecules of RNA that infect plants
C) viral DNA that has had to attach itself to the host genome
D) viruses that invade bacteria
E) a mobile segment of DNA

F) None of the above
G) A) and B)

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Most molecular biologists think that viruses originated from fragments of cellular nucleic acid. Which of the following observations supports this theory?


A) Viruses contain either DNA or RNA.
B) Viruses are enclosed in protein capsids rather than plasma membranes.
C) Viruses can reproduce only inside host cells.
D) Viruses can infect both prokaryotic and eukaryotic cells.
E) Viral genomes are usually more similar to the genome of the host cell than to the genomes of viruses that infect other cell types.

F) All of the above
G) D) and E)

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If you already knew that the infectious agent was either bacterial or viral, which treatment would allow you to distinguish between these two possibilities?


A) I
B) II
C) III
D) IV
E) either II or IV

F) C) and E)
G) B) and C)

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Use the following information to answer the following questions. In 1971, David Baltimore described a scheme for classifying viruses based on how the virus produces mRNA. The table below shows the results of testing five viruses for nuclease specificity, the ability of the virus to act as an mRNA, and presence (+) or absence (-) of its own viral polymerase.  Virus Nuclease  Sensitivity  Genome as mRNA PolymeraseA Dnase +B Rnase +C Dnase +D Rnase +E Rnase +\begin{array}{ll}\text { Virus Nuclease } & \text { Sensitivity } &\text { Genome as mRNA}&\text { Polymerase} \\A & \text { Dnase }&-&+ \\\mathrm{B} & \text { Rnase }&+&- \\\mathrm{C} & \text { Dnase } &-&+\\\mathrm{D} & \text { Rnase }&-&+ \\\mathrm{E} & \text { Rnase }&+&-\end{array} -Based on the above table, which virus meets the Baltimore requirements for a retrovirus?

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