Question 1

Which of the following places the steps in the PCR procedure in the correct order?
1) Incubate at 94°C to denature DNA strands;
2) Incubate at 72°C for DNA synthesis;
3) Incubate at 60°C for primer hybridization.

Accepted Answer

A)  1,2,3
B)  3,2,1
C)  1,3,2
D)  2;1;3
E)  3;1;2F) B) and D)
G) C) and D)

Question 2

Figure 9.5
 
-In Figure 9.5,the marker genes used for selecting recombinant DNA are

Accepted Answer

A)  HindIII,BamHI,and EcoRI.
B)  ampᴿ and lacZ.
C)  ori.
D)  ampᴿ and ori.
E)  lacZ and ori.F) B) and E)
G) A) and D)

Question 3

Your research group is investigating the possible use of genetically-engineered cells to produce a vaccine for malaria.List the pros and cons of using E.coli,Saccharomyces,and animal cells to produce the vaccine.

Accepted Answer

Pros and cons of using E. coli, Saccharo...

Question 4

The Human Genome Project,which was completed in 2003,was focused on

Accepted Answer

A)  identifying all of the genes in the human genome.
B)  determining the nucleotide sequence of the entire human genome.
C)  determining all of the proteins encoded by the human genome.
D)  finding a cure for all human genetic disorders.
E)  cloning all of the genes of the human genome.F) C) and D)
G) A) and B)

Question 5

Nearly all cells,including E.coli and yeast,naturally take up DNA from their surroundings without chemical treatment.

Accepted Answer

A) True 
 B)False

Question 6

111111
-Which enzyme would cut this strand of DNA? GCATGGATCCCAATGC
A)

B)

C)

D)

E)

Accepted Answer

The answer of 111111
-Which enzyme would cut this strand of...

Question 7

An advantage of using Saccharomyces cerevisiae over Escherichia coli to produce a human protein is that

Accepted Answer

A)  it will be more likely to continuously secrete the protein.
B)  it will be less likely to express the protein.
C)  it has a genome only 4 times larger than E.coli.
D)  its genome is well understood unlike E.coli.
E)  it can be engineered to produce human proteins unlike E.coli.F) D) and E)
G) C) and E)

Question 8

The following steps must be performed to make a bacterium produce human protein X.
1-Translation
2-Restriction enzyme
3-Prokaryotic transcription
4-DNA ligase
5-Transformation
6-Eukaryotic transcription
7-Reverse transcription
Which of the following places the steps in the correct order?

Accepted Answer

A)  5,2,3,4,7,6,1
B)  1,2,3,5,4,7,6
C)  6,7,2,3,4,5,1
D)  6,7,2,4,5,3,1
E)  6,2,1,3,4,5,7F) All of the above
G) B) and D)

Question 9

Which of the following techniques is NOT used to introduce recombinant DNA into plants?

Accepted Answer

A)  gene guns
B)  protoplast fusion
C)  Ti plasmids and Agrobacterium
D)  microinjection
E)  electroporationF) None of the above
G) C) and D)

Question 10

You have a small gene that you wish replicated by PCR.After 3 replication cycles,how many double-stranded DNA molecules do you have?

Accepted Answer

A)  2
B)  4
C)  8
D)  16
E)  thousandsF) A) and C)
G) None of the above

Question 11

In recombinant DNA technology,a vector is a self-replicating segment of DNA,such as a plasmid or viral genome.

Accepted Answer

A) True 
 B)False

Question 12

The local public health agency has received reports of an outbreak of Salmonella gastroenteritis among attendees at a city-sponsored chili cook-off.What techniques from recombinant DNA technology would the agency likely use to investigate this outbreak? Describe the expected results from these techniques.

Accepted Answer

The local public health agency would lik...

Question 13

An advantage of cDNA over genomic DNA is that it

Accepted Answer

A)  lacks exons.
B)  lacks introns.
C)  contains selectable markers.
D)  can form very large DNA segments.
E)  is very easy to isolate.F) A) and B)
G) A) and E)

Question 14

The reaction catalyzed by reverse transcriptase is

Accepted Answer

A)  DNA → mRNA.
B)  mRNA → cDNA.
C)  mRNA → protein.
D)  DNA → DNA.
E)  tRNA → mRNA.F) B) and C)
G) All of the above

Question 15

Biotechnology involves the

Accepted Answer

A)  use of microorganisms to make desired products.
B)  use of animal cells to make vaccines.
C)  development of disease-resistant crop plants.
D)  use of microorganisms to make desired products and the use of animal cells to make vaccines.
E)  use of microorganisms to make desired products,the use of animal cells to make vaccines,and the development of disease-resistant crop plants.F) B) and C)
G) A) and E)

Question 16

The restriction enzyme EcoRI recognizes the sequence G↓AATTC.Which of the following is TRUE of DNA after it is treated with EcoRI?

Accepted Answer

A)  All of the DNA fragments will have single-stranded regions ending in AA.
B)  All of the DNA fragments will have single-stranded regions ending in G.
C)  Some of the DNA will have single-stranded regions ending in AA and others will end in G.
D)  All of the DNA will have blunt ends.
E)  All of the DNA will be circular.F) C) and E)
G) A) and D)

Question 17

Figure 9.5
 
-Which of the following methods would be used to introduce the plasmid shown in Figure 9.5 into E.coli?

Accepted Answer

A)  microinjection
B)  transformation
C)  gene guns
D)  Ti plasmids and AgrobacteriumE) None of the above
F) B) and C)

Question 18

The term biotechnology refers exclusively to the use of genetically engineered organisms for the production of desired products.

Accepted Answer

A) True 
 B)False

Question 19

If you have inserted a gene in the Ti plasmid,the next step in genetic engineering is

Accepted Answer

A)  transformation of E.coli with Ti plasmid.
B)  splicing T DNA into a plasmid.
C)  transformation of an animal cell.
D)  inserting the Ti plasmid into Agrobacterium.
E)  inserting the Ti plasmid into a plant cell.F) D) and E)
G) B) and E)

Question 20

The field of proteomics concerns itself with

Accepted Answer

A)  identifying an organism that makes improved enzymes.
B)  discovering the function of genes from a genetic sequence.
C)  determining all of the proteins expressed by a cell.
D)  synthesizing proteins from altered genetic sequences.
E)  identifying pathogens using RFLPs.F) A) and B)
G) A) and C)

Exam 9: Biotechnology and Dna Technology

The reaction catalyzed by reverse transcriptase is

Correct Answer
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The following steps must be performed to make a bacterium produce human protein X. 1-Translation 2-Restriction enzyme 3-Prokaryotic transcription 4-DNA ligase 5-Transformation 6-Eukaryotic transcription 7-Reverse transcription Which of the following places the steps in the correct order?

Correct Answer
verified

Biotechnology involves the

Correct Answer
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The term biotechnology refers exclusively to the use of genetically engineered organisms for the production of desired products.

Correct Answer
verified

Correct Answer
Answered by ExamLex AI
The local public health agency would lik...
View Answer

Figure 9.5 -In Figure 9.5,the marker genes used for selecting recombinant DNA are

Correct Answer
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Figure 9.5 -Which of the following methods would be used to introduce the plasmid shown in Figure 9.5 into E.coli?

Correct Answer
verified

Which of the following places the steps in the PCR procedure in the correct order? 1) Incubate at 94°C to denature DNA strands; 2) Incubate at 72°C for DNA synthesis; 3) Incubate at 60°C for primer hybridization.

Correct Answer
verified

The Human Genome Project,which was completed in 2003,was focused on

Correct Answer
verified

Which of the following techniques is NOT used to introduce recombinant DNA into plants?

Correct Answer
verified

In recombinant DNA technology,a vector is a self-replicating segment of DNA,such as a plasmid or viral genome.

Correct Answer
verified

Your research group is investigating the possible use of genetically-engineered cells to produce a vaccine for malaria.List the pros and cons of using E.coli,Saccharomyces,and animal cells to produce the vaccine.

Correct Answer
Answered by ExamLex AI
Pros and cons of using E. coli, Saccharo...
View Answer

The restriction enzyme EcoRI recognizes the sequence G↓AATTC.Which of the following is TRUE of DNA after it is treated with EcoRI?

Correct Answer
verified

You have a small gene that you wish replicated by PCR.After 3 replication cycles,how many double-stranded DNA molecules do you have?

Correct Answer
verified

The field of proteomics concerns itself with

Correct Answer
verified

An advantage of using Saccharomyces cerevisiae over Escherichia coli to produce a human protein is that

Correct Answer
verified

If you have inserted a gene in the Ti plasmid,the next step in genetic engineering is

Correct Answer
verified

Nearly all cells,including E.coli and yeast,naturally take up DNA from their surroundings without chemical treatment.

Correct Answer
verified

111111 -Which enzyme would cut this strand of DNA? GCATGGATCCCAATGC A) B) C) D) E)

Correct Answer
verified

An advantage of cDNA over genomic DNA is that it

Correct Answer
verified

Exam 9: Biotechnology and Dna Technology

The reaction catalyzed by reverse transcriptase is

Correct AnswerverifiedShow Answer

The following steps must be performed to make a bacterium produce human protein X. 1-Translation 2-Restriction enzyme 3-Prokaryotic transcription 4-DNA ligase 5-Transformation 6-Eukaryotic transcription 7-Reverse transcription Which of the following places the steps in the correct order?

Correct AnswerverifiedShow Answer

Biotechnology involves the

Correct AnswerverifiedShow Answer

The term biotechnology refers exclusively to the use of genetically engineered organisms for the production of desired products.

Correct AnswerverifiedShow Answer

Correct AnswerAnswered by ExamLex AIThe local public health agency would lik...View AnswerShow Answer

Figure 9.5 -In Figure 9.5,the marker genes used for selecting recombinant DNA are

Correct AnswerverifiedShow Answer

Figure 9.5 -Which of the following methods would be used to introduce the plasmid shown in Figure 9.5 into E.coli?

Correct AnswerverifiedShow Answer

Which of the following places the steps in the PCR procedure in the correct order? 1) Incubate at 94°C to denature DNA strands; 2) Incubate at 72°C for DNA synthesis; 3) Incubate at 60°C for primer hybridization.

Correct AnswerverifiedShow Answer

The Human Genome Project,which was completed in 2003,was focused on

Correct AnswerverifiedShow Answer

Which of the following techniques is NOT used to introduce recombinant DNA into plants?

Correct AnswerverifiedShow Answer

In recombinant DNA technology,a vector is a self-replicating segment of DNA,such as a plasmid or viral genome.

Correct AnswerverifiedShow Answer

Your research group is investigating the possible use of genetically-engineered cells to produce a vaccine for malaria.List the pros and cons of using E.coli,Saccharomyces,and animal cells to produce the vaccine.

Correct AnswerAnswered by ExamLex AIPros and cons of using E. coli, Saccharo...View AnswerShow Answer

The restriction enzyme EcoRI recognizes the sequence G↓AATTC.Which of the following is TRUE of DNA after it is treated with EcoRI?

Correct AnswerverifiedShow Answer

You have a small gene that you wish replicated by PCR.After 3 replication cycles,how many double-stranded DNA molecules do you have?

Correct AnswerverifiedShow Answer

The field of proteomics concerns itself with

Correct AnswerverifiedShow Answer

An advantage of using Saccharomyces cerevisiae over Escherichia coli to produce a human protein is that

Correct AnswerverifiedShow Answer

If you have inserted a gene in the Ti plasmid,the next step in genetic engineering is

Correct AnswerverifiedShow Answer

Nearly all cells,including E.coli and yeast,naturally take up DNA from their surroundings without chemical treatment.

Correct AnswerverifiedShow Answer

111111 -Which enzyme would cut this strand of DNA? GCATGGATCCCAATGC A) B) C) D) E)

Correct AnswerverifiedShow Answer

An advantage of cDNA over genomic DNA is that it

Correct AnswerverifiedShow Answer

Correct Answer
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Correct Answer
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Correct Answer
verified

Correct Answer
verified

Correct Answer
Answered by ExamLex AI
The local public health agency would lik...
View Answer

Correct Answer
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Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
Answered by ExamLex AI
Pros and cons of using E. coli, Saccharo...
View Answer

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
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Correct Answer
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Correct Answer
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Correct Answer
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Correct Answer
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